嘉 宾：RANDOLPH V. LEWIS USTAR Professor of Biology
题 目： Structure/Function of Spider Silk Proteins and High Performance
Liquid Chromatography of Proteins and Peptides
时 间: 2014年10月12日（周日）上午9:00-10:00
地 点: 新威尼斯v0008独墅湖校区403-3120
邀请人: 应用生物系贡成良教授
演讲嘉宾简介：
PROFESSIONAL EXPERIENCE
1978-1980 Postdoctoral Roche Inst. of Mol. Biol.
1980-1984 Asst. Prof. University of Wyoming
1984-1989 Assoc. Prof. University of Wyoming
1986-1991 Dept. Head University of Wyoming
1989-2011 Professor University of Wyoming
1990-2011 Dir. Wyoming NSF EPSCoR program
1994-2011 President WyoBiGen
2003-2011 Board of Directors Wyoming Technology Business Center
2011- USTAR Professor of Biology, Utah State University
RESEARCH INTERESTS:
The major research effort is directed toward determining the amino acid sequences of the proteins that comprise different spider silks and correlating these sequences to the unique physical properties of these silk fibers. Additional interests are in the structure/function relationships of proteins.
PUBLICATIONS - REFEREED JOURNALS: (from 2006- )
1. (2006) Lewis, R.V. Spider Silk: Ancient Ideas for New Biomaterials. Biomaterials, Chemical Rev. 106(9): 3762-3774
2. (2006) Brooks, A.E. and Lewis, R.V. Probing the elastic nature of spider silk in pursuit of the next designer fiber, Chemical Technology May, 12-14.
3. (2007) Florence Teulé, Furin W.A., Cooper A.R., Duncan J.R., and Lewis R.V. Modifications of spider silk sequences in an attempt to control the mechanical properties of the synthetic fibers J. of Materials Sciences 42:8974–8985.
4. (2007) Brooks, A.E., Brothers, T.J., Creager, M.S., Lewis R.V. A novel methodology to explore the viscoelasticity of spider major ampullate silk. J. of Applied Biomaterials and Biomechanics 3:158-165.
5. (2007) Jessica E. Garb, Teresa DiMauro, Randolph V. Lewis, and Cheryl Y. Hayashi Expansion and Intragenic Homogenization of Spider Silk Genes since the Triassic: Evidence from Mygalomorphae (Tarantulas and Their Kin) Spidroins Molecular Biology and Evolution: 24(11):2454-2464.
6. (2007) Bittencourt, D., Souto, B.M., Verza, N.C., Vinecky, F., Dittmar, K., Silva, P.I. Jr, Andrade, A.C., da Silva, F.R., Lewis, R.V., Rech, E.L. Spidroins from the Brazilian spider Nephilengys cruentata (Araneae: Nephilidae). Comp. Biochem. Physiol. B Biochem. Mol. Biol. 4, 597-606.
7. (2008) Gregory P. Holland, Melinda S. Creager, Janelle E. Jenkins, Randolph V. Lewis, and Jeffery L. Yarger, Determining Secondary Structure in Spider Dragline Silk Using Carbon-Carbon Correlation Solid-State NMR Spectroscopy, J. Am. Chem. Soc., 2008, 130, 9871–9877.
8. (2008) Gregory P. Holland, Janelle Jenkins, Melinda Creager, Randolph V. Lewis and Jeffery Yarger. Solid State Investigation of Major and Minor Ampullate Spider Silk in the Native and Hydrated States Biomacromolecules 9: 651–657.
9. (2008) Properties of synthetic spider silk fibers based on Argiope aurantia MaSp 2, Amanda E. Brooks, Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C.Russell Middaugh, Randolph V. Lewis. Biomacromolecules 9: 1506-1510
10. (2008) Gregory P. Holland, Janelle E. Jenkins, Melinda S. Creager, Randolph V. Lewis, and Jeffery L. Yarger. Quantifying the fraction of glycine and alanine in β-sheet and helical conformations in spider dragline silk with solid-state NMR, Chemical Communications, 2008, 5568 – 5570 (also selected for publication in Chemical Biology Research Articles, a virtual journal)
11. (2008) Benjamin D. Brooks, Amy E. Albertson, Justin A. Jones, Jonathan O. Speare, Randolph V.Lewis. Efficient screening of high-signal and low-background antibody pairs in the bio-bar code assay using prion protein as the target, Analytical Biochemistry 382: 60-62
12. (2008) Amanda Brooks, Shane R. Nelson, Justin A. Jones, Courtney Koenig, Michael Hinman, Shane Stricker and Randolph V. Lewis, Distinct contributions of model MaSp1 and MaSp2 Like peptides to the mechanical properties of synthetic major ampullate silk fibers as revealed in silico, Nanotechnology, Science and Applications 1:9-16
13. (2009) Florence Teulé, Alyssa R Cooper, William A Furin, Daniela Bittencourt, Elibio L Rech, Amanda Brooks and Randolph V Lewis. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nature Protocols:4, 341-355
14. (2009) Brooks, Benjamin and Lewis, Randolph V. Identification of Problems Developing an Ultrasensitive Immunoassay for the Ante Mortem Detection of the Infectious Isoform of the CWDAssociated Prion Protein, Journal of Immunoassay and Immunochemistry, 30: 135–139
15. (2009) AE Brooks, BD Brooks, MS Creager, and RV Lewis. Analyzing the clustering effects of major ampullate silk mechanical properties. Biomed Sci Instrum 45: 232-7.
16. (2009) Omer Choresh, Battuya Bayarmagnai, and Randolph V. Lewis, Spider Web Glue: Two Proteins Expressed from Opposite Strands of the Same DNA Sequence. Biomacromolecules, 2009, 10, 2852–2856
17. (2010) Janelle E. Jenkins, Melinda S. Creager, Randolph V. Lewis, Gregory P. Holland, Jeffery L. Yarger. Quantitative Correlation Between the Protein Primary Sequences and Secondary Structures in Spider Dragline Silks. Biomacromolecules 11:192-200
18. (2010) Daniela Bittencourt, Katharina Dittmar, Randolph V. Lewis, Elíbio L. Rech. A MaSp2-like gene found in the Amazon mygalomorph spider Avicularia juruensis. Comparative Biochemistry and Physiology, Part B 155 419–426
19. (2010) Creager, Melinda; Jenkins, Janelle; Thagard, Leigh; Brooks, Amanda; Jones, Justin; Lewis, Randy; Holland, Gregory; Yarger, Jeffery, Solid-State NMR Comparison of Various Spiders’ Dragline Silk Fiber. Biomacromolecules 11: 2039-2043
20. (2010) Janelle E. Jenkins, Melinda S. Creager, Emily B. Butler, Randolph V. Lewis, Jeffery L. Yarger. Gregory P. Holland. Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk, Chemical Communications 46: 6714-6716
21. (2010) David J. Perry, Daniela Bittencourt, Elibio L. Rech, and Randolph V. Lewis, Characterization of Piriform Silk Sequences from Three Species of Spiders, Biomacromolecules 11:3000-3006
22. (2011) Melinda S. Creager, Thomas Izdebski, Amanda E. Brooks, Randolph V. Lewis, Elucidating Metabolic Pathways for Amino Acid Incorporation Into Dragline Spider Silk using 13C Enrichment and Solid State NMR, Comparative Biochemistry and Physiology, Part A (in press)
23. (2011) Bo An, Michael B. Hinman, Gregory P. Holland, Jeffery L. Yarger, and Randolph V. Lewis, Inducing β-Sheets Formation in Synthetic Spider Silk Fibers by Aqueous Post-Spin Stretching, Biomacromolecules: 12 (6), pp 2375–2381
24. (2011) Florence Teulé, Bennett Addison, Alyssa R. Cooper, Joel Ayon, Robert W. Henning, Chris J. Benmore, Gregory P. Holland, Jeffery L. Yarger, Randolph V. Lewis, Combining flagelliform and dragline spider silk motifs to produce tunable synthetic biopolymer fibers, (e published, Biopolymers)
25. (2012)Florence Teulé, Yun-Gen Miao, Bong-Hee Sohn, Young-Soo Kim, J. Joe Hull, Malcolm J. Fraser, Jr., Randolph V. Lewis, and Donald L. Jarvis., Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc Natl Acad Sci U S A. 2012; 109(3):923-8.
26. (2012) H. B. Steinkraus, H. Rothfuss, J. A. Jones, E. Dissen, E. Shefferly and R. V. Lewis, The absence of detectable fetal microchimerism in nontransgenic goats (Capra aegagrus hircus) bearing transgenic offspring, J. of Animal Science 90(2): 481-488