报告时间：2011年11月11日（周五）下午14：00-15：30
报告地点：新威尼斯v0008独墅湖校区医学楼403-3119会议室
报告一：Signaling spread through plasmodesmata and its regulation
报告内容简介：
Cell-to-cell communication is a pivotal process in the determination of cell fate during development and physiological adaptation in response to environmental stimuli. The intercellular movement of proteins and RNAs in addition to the movement of phytohormones has emerged as a novel mechanism of cell-to-cell signaling in plants. As a strategy for efficient intercellular communication, plants have evolved plant-specific symplasmic communication networks via plasmodesmata (PD). These processes play an important role to regulate the plant growth and development. However, mechanism of PD regulation and machineries of macromolecule trafficking are still largely unknown. We used reverse genetic approaches to identify callose synthase that control callose accumulation at PD, resulting in the regulation of PD pore size. We showed that PD regulation is tightly controlled by a callose synthase, AtGSL8, of which function is required for auxin gradient during tropism. We will also introduce our research interest related to plasmodesmal biology.
报告人及其简介：
Jae-Yean Kim, Ph.D., Associate Professor of Gyeongsang National University
近三年发表的论文：
1.Xu et al (2011) Chaperonins facilitate KNOTTED1 cell-to-cell trafficking and stem cell function. Science 333: 1141-1144
2.Jo et al (2011) Plasmodesmal receptor-like kinases identified through analysis of a rice cell wall proteomics database. Protoplasma (Special issue for Plasmodesmata) 248(1): 101-116
3.Hyun et al (2011) Cell-to-cell trafficking of RNA and RNA silencing through plasmodesmata. Protoplasma 248(1): 191-203 (invited review).
4.Lucas et al (2009) Plasmodesmata-bridging the gaps between neighboring plant cells. Trend in Cell Biol 19(10): 495-503 (invited review).
5.Huang et al (2009) The genome of the cucumber, Cucumis sativus L. Nature Genetics 41(12): 1275-1281
6.Spensley et al (2009) Evolutionarily conserved regulatory motifs in the promoter of the Arabidopsis clock gene LHY. Plant Cell 21(9): 2606-2623
7.Chen et al (2009) The Arabidopsis callose synthase gene, GSL8, is required for cytokinesis and cell patterning. Plant Physiol, 2009. 150: p. 105-113.
8.Rim et al (2009) A Non-cell Autonomous Mechanism for Control of Plant Architecture and Epidermal Differentiation Involves Intercellular Trafficking of BREVIPEDICELLUS. Functional Plant Biol 36: 280-289.
报告二：Metabolic engineering of E. coli as a platform strain for isoprenoids production
报告内容简介：
Isoprenoids, also called terpenoids, are a large and diverse class of naturally-occurring compounds. They are present in all living organisms and include many important drugs, valuable flavor and fragrance compounds, pigments, antioxidants, steroids and natural polymers. They are derived from five-carbon universal building blocks assembled and modified in various ways. IPP (isopentenyl diphosphate) and DMAPP (dimethylallyl diphosphate) are the building blocks which are produced from either the mevalonic acid (MVA) or methylerythritol phosphate (MEP) pathways. The increased synthesis of building blocks of IPP and DMAPP through metabolic engineering is a way to enhance the production of terpenoids. Using E. coli as a host, IPP and DMAPP supply can be increased significantly through the introduction of foreign MVA (mevalonate) pathway into it. The MVA pathway is split into two parts with the top and bottom portions supplying mevalonate from acetyl-CoA, and IPP and DMAPP from mevalonate, respectively. The E. coli harboring the bottom MVA pathway of S. pneumoniae and the top MVA pathway of E. faecalis produced the highest amount of carotenoids. The carotenoids are tetraterpenoid pigment molecules, facilitating metabolic engineering for the pathway optimization because of their convenient colorimetric screening properties. Because the root of all terpenoids synthesis pathway share the universal C5 metabolic precursors (IPP and DMAPP), metabolic engineering work with carotenoids can provide genetic platforms for the production of other valuable isoprenoids. We successfully produced the valuable compounds of coenzyme Q10 and retinoids using the platform E. coli strain resulting from the carotenoids works.
报告人及其简介：
Seon-Won Kim，Ph.D.,Professor, Division of Applied Life Science, Gyeongsang National University
近三年发表的主要论文：
1.Lijun Huang; Xiong-Yan Chen; Yeonggil Rim; Xiao Han; Won Kyong Cho; Seon-Won Kim; Jae-Yean Kim “Arabidopsis Glucan Synthase-Like 10 functions in male gametogenesis” Journal of Plant Physiology, 166(4): 344-352 (2009-3-1) SCI (IF=2.437)
2.EG Lee, SH Yoon, A Das, SH Lee, C Li, JY Kim, MS Choi, DK Oh, and SW Kim “Directing vanillin production from ferulic acid by increased acetyl-CoA consumption in recombinant E. coli” Biotechnol. Bioeng. 102(1): 200-208 (2009-1-1) SCI (IF=2.936)
3.Xiong-Yan Chen, Sun-Tae Kim, Won-Kyong Cho, Yeonggil Rim, Suwha Kim, Seon-Won Kim, Kyu-Young Kang, Zee-Yong Park, Jae-Yean Kim, “Proteomics of weakly-bound cell wall proteins in rice calli” Journal of Plant Physiology, 166(7): 675-685 (2009-5-1) SCI (IF=2.437)
4.WK Cho, XY Chen, HS Chu, YG Rim, SW Kim, ST Kim, SW Kim, ZY Park, JY Kim, “Proteomic analysis of the secretome of rice calli” Physiologia Plantarum, 135(4): 331-341 (2009-4) SCI (IF=2.334)
5.S.H. Yoon, S.H. Lee, A. Das, H.K. Ryu, H.J. Jang, J.Y. Kim, D.K. Oh, Jay D Keasling; S.W. Kim, “Combinatorial expression of bacterial whole mevalonate pathway for the production of b-carotene in E. coli” J. Biotechnol. 140(3-4):218-226 (2009-03-25) SCI (IF=2.970)
6.WK Cho, XY Chen, NM Uddina, YG Rim, JY Moon, JH Jung, CL Shi, HS Chu, SW Kim, SW Kim, ZY Park, JY Kim, “Comprehensive proteome analysis of lettuce latex using multidimensional protein identification technology” Phytochemistry, 70(5): 570-578 (2009-3) SCI (IF=2.946)
7.XY Chen, L Liu, EK Lee, X Han, YG Rim, HS Chu, SW Kim, F Sack, JY Kim, “The Arabidopsis callose synthase gene, GSL8, is required for cytokinesis and cell patterning” Plant Physiology 150(1): 105-113 (2009-05) SCI (IF=6.110)
8.M Yasir, Z Aslam, SW Kim, SW Lee, CO Jeon, YR Chung, “Bacterial community composition and chitinase gene diversity of vermicompost with antifungal activity” Bioresour. Technol., 100(19): 4396–4403 (2009-10) SCI (IF=4.253)
9.YS Kim, NH Kim, SJ Yeom, SW Kim, and DK Oh, “In Vitro Characterization of a Recombinant Blh Protein from an Uncultured Marine Bacterium as a b-Carotene 15,15′-Dioxygenase” Journal of Biological Chemistry, 284(23): 15781–15793 (2009-06-05) SCI (IF=5.520)
10.JH Kim, SW Kim, DQA Nguyen, H Li, SB Kim, YG Seo, JK Yang, IY Chung, DH Kim, and CJ Kim, “Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures” Biotechnol Bioprocess Eng., 14: 559-564 (2009-12-01) SCIE (IF=1.412)
11.Z. Aslam, J.-H. Park, S.W. Kim, C.O. Jeon and Y. R. Chung, “Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov.” Int J Syst Evol Microbiol., 59(12): 2967-2972 (2009-12-01) SCI (IF=2.222)
12.WK Cho, XY Chen, YG Rim, HS Chu, SW Kim, SW Kim, ZY Park, and JY Kim, “Proteome study of the phloem sap of pumpkin using multidimensional protein identification technology” Journal of Plant Physiology, 167:771-778 (2010-07-01) SCI (IF=2.437)
13.CL Wang, SH Yoon, AA Shah, YR Chung, JY Kim, ES Choi, JD Keasling, SW Kim, “Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway” Biotechnol. Bioeng. 107(3): 421-429 (2010-10-15) SCI (IF=3.377)
14.CL Wang, JY Kim, ES Choi, and SW Kim, “Microbial Production of Farnesol (FOH): Current states and Beyond” Process Biochemistry, 46(6): 1221-1229 (2011-06) (IF=2.444)
15.CL Wang, SH Yoon, HJ Jang, YR Chung, JY Kim, ES Choi, and SW Kim, “Metabolic Engineering of Escherichia coli for α-Farnesene Production” Metabolic Engineering, doi:10.1016/j.ymben.2011.08.001 (2011-08-20) (IF=5.512)
16.HJ Jang, SH Yoon, HK Ryu, JH Kim, CL Wang, JY Kim, DK Oh and SW Kim “Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system” Microbial Cell Factories, 10(59): 1-12, doi:10.1186/1475-2859-10-59 (2011-07-29) (IF=4.540)
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